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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB1587 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-28A/IFN-lambda 2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-28A/IFN-lambda 2 in direct ELISAs. In direct ELISAs, 83% cross-reactivity with recombinant human (rh) IL-28B/IFN-lambda 3 and no cross-reactivity with recombinant mouse IL-28B/IFN-lambda 3 or rhIL-29/IFN-l1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8IZJ0- Isotype
- IgG
- Antibody clone number
- 248526
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Type III IFNs are produced by and stimulate human plasmacytoid dendritic cells.
Yin Z, Dai J, Deng J, Sheikh F, Natalia M, Shih T, Lewis-Antes A, Amrute SB, Garrigues U, Doyle S, Donnelly RP, Kotenko SV, Fitzgerald-Bocarsly P
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 15;189(6):2735-45
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 15;189(6):2735-45
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-28A/IFN-lambda 2 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IL-28A/IFN-lambda 2 Antibody. Recombinant Human IL-28A/IFN-lambda 2 (Catalog # 1587-IL) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recom-binant Human IL-28A/IFN-lambda 2 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-28A/IFN-lambda 2 Monoclonal Antibody (Catalog # MAB1587). The ND50 is typically 0.25-1.0 µg/mL.
