Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-87056 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-29 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-29 in various cell lines. Sample was incubated with IL-29 polyclonal antibody (Product # PA5-87056) using a 1:1000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IL-29 Polyclonal Antibody (Product # PA5-87056) and a 22 kDa band corresponding to Interferon lambda-1 was in the treatment panel tested. No band was observed in U-937 cells treated with protein transport inhibitor which is a negative expresser of IL-29. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 differentiated to M2 macrophages treated with Human IFN Alpha, Lipopolysaccharide and Protein transport inhibitor (Lane 2), THP-1 differentiated to M2 macrophages treated with Human IFN Alpha, Polyinosinic–polycytidylic acid sodium salt and Protein transport inhibitor (Lane 3), THP-1 treated with Protein transport inhibitor (Lane 4), U-937 (Lane 5) and U-937 treated with Protein transport inhibitor (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) at ~70 kDa was also observed in a few cell lines.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IL-29 in paraffin-embedded rat lung. Sample was incubated with IL-29 polyclonal antibody (Product # PA5-87056) using a 1:100 dilution. Images are presented at 40x magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IL-29 in paraffin-embedded rat brain. Sample was incubated with IL-29 polyclonal antibody (Product # PA5-87056) using a 1:100 dilution. Images are presented at 40x magnification.