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Western blot
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- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00057154-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00057154-M01, RRID:AB_2193839
- Product name
- Mouse Monoclonal SMURF1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SMURF1 (1D7)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Role of SMURF1 ubiquitin ligase in BMP receptor trafficking and signaling.
Generation of Smurf2 Conditional Knockout Mice.
Non-proteolytic ubiquitin modification of PPARγ by Smurf1 protects the liver from steatosis.
Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial Hypertension.
Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer.
The E3 ubiquitin ligase SMAD ubiquitination regulatory factor 2 negatively regulates Krüppel-like factor 5 protein.
Smurf1 ubiquitin ligase causes downregulation of BMP receptors and is induced in monocrotaline and hypoxia models of pulmonary arterial hypertension.
Murakami K, Etlinger JD
Cellular signalling 2019 Feb;54:139-149
Cellular signalling 2019 Feb;54:139-149
Generation of Smurf2 Conditional Knockout Mice.
Tang Y, Tang LY, Xu X, Li C, Deng C, Zhang YE
International journal of biological sciences 2018;14(5):542-548
International journal of biological sciences 2018;14(5):542-548
Non-proteolytic ubiquitin modification of PPARγ by Smurf1 protects the liver from steatosis.
Zhu K, Tang Y, Xu X, Dang H, Tang LY, Wang X, Wang XW, Zhang YE
PLoS biology 2018 Dec;16(12):e3000091
PLoS biology 2018 Dec;16(12):e3000091
Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial Hypertension.
Barnes JW, Kucera ET, Tian L, Mellor NE, Dvorina N, Baldwin WW 3rd, Aldred MA, Farver CF, Comhair SA, Aytekin M, Dweik RA
American journal of respiratory cell and molecular biology 2016 Oct;55(4):564-575
American journal of respiratory cell and molecular biology 2016 Oct;55(4):564-575
Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer.
Wang J, Zhang Y, Weng W, Qiao Y, Ma L, Xiao W, Yu Y, Pan Q, Sun F
The Journal of biological chemistry 2013 Nov 22;288(47):33667-33681
The Journal of biological chemistry 2013 Nov 22;288(47):33667-33681
The E3 ubiquitin ligase SMAD ubiquitination regulatory factor 2 negatively regulates Krüppel-like factor 5 protein.
Du JX, Hagos EG, Nandan MO, Bialkowska AB, Yu B, Yang VW
The Journal of biological chemistry 2011 Nov 18;286(46):40354-64
The Journal of biological chemistry 2011 Nov 18;286(46):40354-64
Smurf1 ubiquitin ligase causes downregulation of BMP receptors and is induced in monocrotaline and hypoxia models of pulmonary arterial hypertension.
Murakami K, Mathew R, Huang J, Farahani R, Peng H, Olson SC, Etlinger JD
Experimental biology and medicine (Maywood, N.J.) 2010 Jul;235(7):805-13
Experimental biology and medicine (Maywood, N.J.) 2010 Jul;235(7):805-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody (M01), clone 1D7.Lane 1: SMURF1 transfected lysate(83.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SMURF1 Antibody (1D7) [H00057154-M01] - Detection limit for recombinant GST tagged SMURF1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 2 ug/ml.
