Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB1746 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1746, RRID:AB_1238120
- Product name
- SMURF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SMURF1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity.
Schwamborn JC, Khazaei MR, Püschel AW
The Journal of biological chemistry 2007 Nov 30;282(48):35259-68
The Journal of biological chemistry 2007 Nov 30;282(48):35259-68
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SMURF1 polyclonal antibody (Cat # PAB1746) is used in Western blot to detect SMURF1 in HL-60 cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SMURF1 polyclonal antibody (Cat # PAB1746) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)