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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB1747 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1747, RRID:AB_1238121
- Product name
- SMURF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SMURF1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts.
Kaneki H, Guo R, Chen D, Yao Z, Schwarz EM, Zhang YE, Boyce BF, Xing L
The Journal of biological chemistry 2006 Feb 17;281(7):4326-33
The Journal of biological chemistry 2006 Feb 17;281(7):4326-33
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SMURF1 polyclonal antibody (Cat # PAB1747) in mouse kidney tissue lysate (35 ug/lane). SMURF1 (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Figure 1. C2C12 or 2T3 cells were treated with PBS or TNF (2.5-7.5 ng/mL) for 72 h. SMURF1 and beta-actin protein levels were detected by Western blot SMURF1 polyclonal antibody (Cat # PAB1747).Figure 2. Proteins were extracted from the metaphyseal region of 4-month-old TNF-over-expressing mice (TNF-Tg) and wild type (wt) littermates (left panel). PBS or TNF was injected (0.25 ug/injection, 3 times/day for 3 days) into the calvarial bones of wt mice. Proteins were extracted from the bones and analyzed by Western blotting for SMURF1 polyclonal antibody (Cat # PAB1747), Runx2 and beta-actin protein levels (right panel).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SMURF1 polyclonal antibody (Cat # PAB1747) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
