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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- LS-C186637 - Provider product page
- Provider
- LSBio
- Product name
- ZNRF1 Antibody (aa93-106) LS-C186637
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Mouse, Bovine, Canine, Guinea Pig, Hamster
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ZNRF1 antibody N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with (1 ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ZNRF1 antibody (10 ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, Streptavidin-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).
