Explore
Validate
Learn
Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A300-173A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Paf1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse Paf1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 (Cat. No. A300-173A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Paf1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Paf1 (Cat. No. A300-173A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody A300-173A (lot A300-173A-3) used for IP at 6 µg per reaction. Paf1 was also immunoprecipitated by a previous lot of this antibody (lot A300-173A-2) and rabbit anti-Paf1 antibody A300-172A-2 For blotting immunoprecipitated Paf1, A300-173A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
