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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-34954 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CFDP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, U87-MG, SK-N-SH, SK-N-AS. Predicted reactivity: Dog (89%), Rhesus Monkey (98%), Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CFDP1 Polyclonal Antibody detects CFDP1 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract. B. 30 µg SK-N-SH whole cell lysate/extract. C. 30 µg SK-N-AS whole cell lysate/extract.12 % SDS-PAGE. CFDP1 Polyclonal Antibody (Product # PA5-34954) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CFDP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CFDP1 Polyclonal Antibody (Product # PA5-34954) diluted at 1:1500. Red: alpha Tubulin, a cytoskeleton marker. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CFDP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CFDP1 Polyclonal Antibody (Product # PA5-34954) diluted at 1:1500. Red: alpha Tubulin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CFDP1 was performed in paraffin-embedded human breast carcinoma tissue using CFDP1 Polyclonal Antibody (Product # PA5-34954) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of CFDP1 was performed in frozen-sectioned mouse mouse cerebellum tissue using CFDP1 Polyclonal Antibody (Product # PA5-34954) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of CFDP1 was performed in frozen-sectioned mouse mouse cerebellum tissue using CFDP1 Polyclonal Antibody (Product # PA5-34954) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CFDP1 was performed in paraffin-embedded human breast carcinoma tissue using CFDP1 Polyclonal Antibody (Product # PA5-34954) at a dilution of 1:1000.
