Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-27809 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CFDP1 Monoclonal Antibody (GT11811)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, U87-MG, SK-N-SH, IMR32, SK-N-AS
- Antibody clone number
- GT11811
- Concentration
- 2.34 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CFDP1 in non-transfected (-) and transfected (+) 293T cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with CFDP1 monoclonal antibody (Product # MA5-27809) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of CFDP1 in A) U87-MG whole cell lysate, B) SK-N-SH whole cell lysate, C) IMR32 whole cell lysate, D) SK-N-AS whole cell lysate using 20 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with CFDP1 monoclonal antibody (Product # MA5-27809) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CFDP1 Monoclonal Antibody (GT11811) detects CFDP1 protein by western blot analysis. A U87-MG whole cell lysate/extract. B SK-N-SH whole cell lysate/extract. C IMR32 whole cell lysate/extract. D SK-N-AS whole cell lysate/extract.12 % SDS-PAGE. CFDP1 Monoclonal Antibody (GT11811) (Product # MA5-27809) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CFDP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CFDP1 Monoclonal Antibody (GT11811) (Product # MA5-27809) diluted at 1:100. Red: alpha Tubulin 4a, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CFDP1 was performed in paraffin-embedded human ovarian cancer tissue using CFDP1 Monoclonal Antibody (GT11811) (Product # MA5-27809) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CFDP1 was performed in paraffin-embedded human breast carcinoma tissue using CFDP1 Monoclonal Antibody (GT11811) (Product # MA5-27809) at a dilution of 1:2000.