RQ6294

antibody from NSJ Bioreagents

Targeting: ATP5MC1 ATP5G, ATP5G1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6294

Provider

NSJ Bioreagents

Product name

ATP5MC1/2/3 Antibody

Antibody type

Monoclonal

Description

This highly specific ATP5MC1/2/3 antibody is suitable for use in Western blot/Flow cytometry applications with human, mouse and rat, monkey samples.

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

12E9.

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the ATP5MC1/2/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HL60, 2) monkey COS-7, 3) rat kidney, 4) rat heart and 5) mouse heart lysate with ATP5MC1/2/3 antibody. Predicted molecular weight: 10-14 kDa.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse HEPA1-6 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of rat RH35 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.