RZ1097

antibody from NSJ Bioreagents

Targeting: ATP5MC1 ATP5G, ATP5G1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RZ1097

Provider

NSJ Bioreagents

Product name

Zebrafish Atp5mc Antibody / Isoforms 1/2/3

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Zebrafish

Host

Rabbit

Vial size

100 ug

Concentration

Lyophilized

Storage

After reconstitution, the Zebrafish Atp5mc antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of Atp5mc1/2/3 protein using Zebrafish Atp5mc antibody and and 1) zebrafish head, 2) female zebrafish viscera, 3) male zebrafish viscera, 4) whole zebrafish and 5) zebrafish embryo tissue lysate. Predicted molecular weight ~10 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Atp5mc antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish brain tissue with Zebrafish Atp5mc antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.