PA5-47859

antibody from Invitrogen Antibodies

Targeting: LY6K CT97, FLJ35226, HSJ001348

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47859

Provider

Invitrogen Antibodies

Product name

LY6K Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human testis tissue. PVDF Membrane was probed with 0.25 µg/mL of Sheep Anti-human Ly6K Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47859) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for Ly6K at approximately 25-27 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LY6K in human testis tissue. Samples were incubated in LY6K polyclonal antibody (Product # PA5-47859) using a dilution of 0.25 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. Specific bands were detected for Ly6K at approximately 25-27 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LY6K in immersion fixed paraffin-embedded sections of human lung cancer tissue. Samples were incubated in LY6K polyclonal antibody (Product # PA5-47859) using a dilution of 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-basic. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane of cancer cells.