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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA1-10035 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Ubiquitin Monoclonal Antibody (Ubi-1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- Ubi-1
- Vial size
- 100 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2.
Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.
Laforin is required for the functional activation of malin in endoplasmic reticulum stress resistance in neuronal cells.
Protein aggregation of SERCA2 mutants associated with Darier disease elicits ER stress and apoptosis in keratinocytes.
A photoconvertible fluorescent reporter to track chaperone-mediated autophagy.
Glucosamine induces autophagy via an mTOR-independent pathway.
Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability.
Regulation of mitochondrial morphology by USP30, a deubiquitinating enzyme present in the mitochondrial outer membrane.
Accelerated Notch-dependent degradation of E47 proteins in aged B cell precursors is associated with increased ERK MAPK activation.
Soft tissue sacrococcygeal chordoma with intracytoplasmic filamentous inclusions.
Morphological, clinical and genetic aspects in a family with a novel LAMP-2 gene mutation (Danon disease).
Decreased E47 in senescent B cell precursors is stage specific and regulated posttranslationally by protein turnover.
NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein.
Cardiac voltage-gated sodium channel Nav1.5 is regulated by Nedd4-2 mediated ubiquitination.
Downregulation of free ubiquitin: a novel mechanism of p53 stabilization and neuronal cell death.
Cyclopentenone prostaglandins of the J series inhibit the ubiquitin isopeptidase activity of the proteasome pathway.
Quantitative neurohistological features of frontotemporal degeneration.
p53 accumulation due to down-regulation of ubiquitin: relevance for neuronal apoptosis.
Human H/ACA small nucleolar RNPs and telomerase share evolutionarily conserved proteins NHP2 and NOP10.
PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia.
Neurofibrillary lesions in experimental aluminum-induced encephalopathy and Alzheimer's disease share immunoreactivity for amyloid precursor protein, A beta, alpha 1-antichymotrypsin and ubiquitin-protein conjugates.
Grindheim AK, Hollås H, Raddum AM, Saraste J, Vedeler A
Journal of cell science 2016 Jan 15;129(2):314-28
Journal of cell science 2016 Jan 15;129(2):314-28
Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.
Niehues S, Bussmann J, Steffes G, Erdmann I, Köhrer C, Sun L, Wagner M, Schäfer K, Wang G, Koerdt SN, Stum M, Jaiswal S, RajBhandary UL, Thomas U, Aberle H, Burgess RW, Yang XL, Dieterich D, Storkebaum E
Nature communications 2015 Jul 3;6:7520
Nature communications 2015 Jul 3;6:7520
Laforin is required for the functional activation of malin in endoplasmic reticulum stress resistance in neuronal cells.
Zeng L, Wang Y, Baba O, Zheng P, Liu Y, Liu Y
The FEBS journal 2012 Jul;279(14):2467-78
The FEBS journal 2012 Jul;279(14):2467-78
Protein aggregation of SERCA2 mutants associated with Darier disease elicits ER stress and apoptosis in keratinocytes.
Wang Y, Bruce AT, Tu C, Ma K, Zeng L, Zheng P, Liu Y, Liu Y
Journal of cell science 2011 Nov 1;124(Pt 21):3568-80
Journal of cell science 2011 Nov 1;124(Pt 21):3568-80
A photoconvertible fluorescent reporter to track chaperone-mediated autophagy.
Koga H, Martinez-Vicente M, Macian F, Verkhusha VV, Cuervo AM
Nature communications 2011 Jul 12;2:386
Nature communications 2011 Jul 12;2:386
Glucosamine induces autophagy via an mTOR-independent pathway.
Shintani T, Yamazaki F, Katoh T, Umekawa M, Matahira Y, Hori S, Kakizuka A, Totani K, Yamamoto K, Ashida H
Biochemical and biophysical research communications 2010 Jan 22;391(4):1775-9
Biochemical and biophysical research communications 2010 Jan 22;391(4):1775-9
Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability.
Pebernard S, Perry JJ, Tainer JA, Boddy MN
Molecular biology of the cell 2008 Oct;19(10):4099-109
Molecular biology of the cell 2008 Oct;19(10):4099-109
Regulation of mitochondrial morphology by USP30, a deubiquitinating enzyme present in the mitochondrial outer membrane.
Nakamura N, Hirose S
Molecular biology of the cell 2008 May;19(5):1903-11
Molecular biology of the cell 2008 May;19(5):1903-11
Accelerated Notch-dependent degradation of E47 proteins in aged B cell precursors is associated with increased ERK MAPK activation.
King AM, Van der Put E, Blomberg BB, Riley RL
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3521-9
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3521-9
Soft tissue sacrococcygeal chordoma with intracytoplasmic filamentous inclusions.
Guarino M, Ballabio G, Rubino B, Nebuloni M, Tosoni A
Pathology, research and practice 2005;201(10):699-704
Pathology, research and practice 2005;201(10):699-704
Morphological, clinical and genetic aspects in a family with a novel LAMP-2 gene mutation (Danon disease).
Lobrinus JA, Schorderet DF, Payot M, Jeanrenaud X, Bottani A, Superti-Furga A, Schlaepfer J, Fromer M, Jeannet PY
Neuromuscular disorders : NMD 2005 Apr;15(4):293-8
Neuromuscular disorders : NMD 2005 Apr;15(4):293-8
Decreased E47 in senescent B cell precursors is stage specific and regulated posttranslationally by protein turnover.
Van der Put E, Frasca D, King AM, Blomberg BB, Riley RL
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):818-27
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):818-27
NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein.
Mori T, Li Y, Hata H, Kochi H
FEBS letters 2004 Jan 16;557(1-3):209-14
FEBS letters 2004 Jan 16;557(1-3):209-14
Cardiac voltage-gated sodium channel Nav1.5 is regulated by Nedd4-2 mediated ubiquitination.
van Bemmelen MX, Rougier JS, Gavillet B, Apothéloz F, Daidié D, Tateyama M, Rivolta I, Thomas MA, Kass RS, Staub O, Abriel H
Circulation research 2004 Aug 6;95(3):284-91
Circulation research 2004 Aug 6;95(3):284-91
Downregulation of free ubiquitin: a novel mechanism of p53 stabilization and neuronal cell death.
Tan Z, Tu W, Schreiber SS
Brain research. Molecular brain research 2001 Jul 13;91(1-2):179-88
Brain research. Molecular brain research 2001 Jul 13;91(1-2):179-88
Cyclopentenone prostaglandins of the J series inhibit the ubiquitin isopeptidase activity of the proteasome pathway.
Mullally JE, Moos PJ, Edes K, Fitzpatrick FA
The Journal of biological chemistry 2001 Aug 10;276(32):30366-73
The Journal of biological chemistry 2001 Aug 10;276(32):30366-73
Quantitative neurohistological features of frontotemporal degeneration.
Arnold SE, Han LY, Clark CM, Grossman M, Trojanowski JQ
Neurobiology of aging 2000 Nov-Dec;21(6):913-9
Neurobiology of aging 2000 Nov-Dec;21(6):913-9
p53 accumulation due to down-regulation of ubiquitin: relevance for neuronal apoptosis.
Tan Z, Qu W, Tu W, Liu W, Baudry M, Schreiber SS
Cell death and differentiation 2000 Jul;7(7):675-81
Cell death and differentiation 2000 Jul;7(7):675-81
Human H/ACA small nucleolar RNPs and telomerase share evolutionarily conserved proteins NHP2 and NOP10.
Pogacić V, Dragon F, Filipowicz W
Molecular and cellular biology 2000 Dec;20(23):9028-40
Molecular and cellular biology 2000 Dec;20(23):9028-40
PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia.
Sternsdorf T, Puccetti E, Jensen K, Hoelzer D, Will H, Ottmann OG, Ruthardt M
Molecular and cellular biology 1999 Jul;19(7):5170-8
Molecular and cellular biology 1999 Jul;19(7):5170-8
Neurofibrillary lesions in experimental aluminum-induced encephalopathy and Alzheimer's disease share immunoreactivity for amyloid precursor protein, A beta, alpha 1-antichymotrypsin and ubiquitin-protein conjugates.
Huang Y, Herman MM, Liu J, Katsetos CD, Wills MR, Savory J
Brain research 1997 Oct 17;771(2):213-20
Brain research 1997 Oct 17;771(2):213-20
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of ubiquitin using Product # MA1-10035. Lane 1 is mono-ubiquitin and polyubiquitin chains. Lane 2 is monoubiquitin only (overloaded). Lanes 3, 4 and 5 are 0.1 mg rat cerebellum, cortex, and brain stem respectively. 20% gels with PVDF blots (Nitrocellulose works but not as well).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ubiquitin (green) in HeLa cells either left untreated (left panel) or treated with 50uM of MG132 proteasome inhibitor (right panel) for 90 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Ubiquitin monoclonal antibody (Product # MA1-10035) at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ubiquitin in hippocampal tissue from an Alzheimer patient, using Product # MA1-10035.
