A-101

antibody from Novus Biologicals

Targeting: RPS27A S27A, Uba80, UBCEP80

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

A-101

Provider

Novus Biologicals

Product name

Rabbit Monoclonal Ubiquitin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. This antibody detects endogenous, human proteins containing K48-linked polyubiquitin chains in Western blots. This antibody detects purified, recombinant K48-linked polyubiquitin chains, but has no cross-reactivity to monoubiquitin or polyubiquitin of other linkages

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 ug

Storage

12 months from date of receipt, -20 degreesC as supplied. 3 months, -20 degreesC under sterile conditions after opening.

References

Submitted references

Validation of Babesia proteasome as a drug target.

Jalovecka M, Hartmann D, Miyamoto Y, Eckmann L, Hajdusek O, O'Donoghue AJ, Sojka D
International journal for parasitology. Drugs and drug resistance 2018 Dec;8(3):394-402

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Ubiquitin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with MG132. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Ubiquitin K48 Linkage Monoclonal Antibody (Catalog # A-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ubiquitin at approximately 75-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot 25 ng of each linkage of recombinant diubiquitin was run on a 10-20% SDS-PAGE gel prior to blotting on PVDF membrane. Western blots were developed using anti-K48 mAb (A-101, upper panel) or anti-ubiquitin mAb (MAB701, lower panel) primaries at 0.5 µg/ml. The appropriate HRP-labeled anti-rabbit or anti-mouse (R&D Systems HAF008 or HAF007) secondary antibodies were used at a 1:2000 dilution. A single band of appropriate size was detected only in the K48-linked diubiquitin lane using A-101.