MAB8595

antibody from R&D Systems

Targeting: RPS27A S27A, Uba80, UBCEP80

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8595

Provider

R&D Systems

Product name

Human Ubiquitin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. Detects human Ubiquitin in direct ELISAs

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

P0CG47


Isotype

IgG

Antibody clone number

1002A

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Ubiquitin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated with MG132. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Ubiquitin at various molecular weights (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Ubiquitin by Western Blot. Western blot shows Di-Ubiquitin chains of the indicated linkage type loaded at 2 ng/lane. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Di-Ubiquitin as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.