Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-96039 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPS27A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPKK NKHKRKKVKL AVLKYYKVDE NGKISRLRRE CPSDECGAGV FMASHFDRHY CGKCCLTYCF NKPEDK; Positive Samples: Jurkat, NIH/3T3, Mouse kidney, Mouse liver, Rat brain; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.98 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of RPS27A in MCF7 cells. Samples were incubated with RPS27A Polyclonal antibody (Product # PA5-96039) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPS27A in paraffin-embedded rat kidney. Samples were incubated with RPS27A Polyclonal antibody (Product # PA5-96039) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPS27A in paraffin-embedded human placenta. Samples were incubated with RPS27A Polyclonal antibody (Product # PA5-96039) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPS27A in paraffin-embedded mouse brain. Samples were incubated with RPS27A Polyclonal antibody (Product # PA5-96039) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.