PA5-77800

antibody from Invitrogen Antibodies

Targeting: GABARAPL2 ATG8, ATG8C, GATE-16, GATE16, GEF2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-77800

Provider

Invitrogen Antibodies

Product name

GABARAPL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GABARAPL2 in rat liver cell lysates with 15 µg of sample. The sample was incubated with GABARAPL2 polyclonal antibody (Product # PA5-77800) using a dilution of 1:1000 (1 hour at 4°C), and followed by Goat Anti-Rabbit HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder (MW), Lane 2: Rat Liver cell lysates.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GABARAPL2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GABARAPL2 polyclonal antibody (Product # PA5-77800) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GABARAPL2 Antibody, D) Merged image. Magnification: 60x.