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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- MAB36791 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LINGO-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human LINGO-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LINGO-1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q7L985- Isotype
- IgG
- Antibody clone number
- 382007
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human LINGO-2 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LINGO-2 Monoclonal Antibody (Catalog # MAB36791) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LINGO-2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LINGO-2 in HEK293 Human Cell Line Transfected with Human LINGO-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line either (A) transfected with human LINGO-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LINGO-2 Monoclonal Antibody (Catalog # MAB36791) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LINGO-2 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Human LINGO-2 Monoclonal Antibody (Catalog # MAB36791, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
