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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-60151 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RC3H2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GAISRSSRTG YHTTDPVQAT ASQGSATKPI SVSDYVPYVN AVDSRWSSYG NEATSSAHYV ERDRFIVTDL SGHRKHSSTG DLLSLELQQA KSNSLLLQ Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 98%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RC3H2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted). Samples were probed using a RC3H2 Polyclonal Antibody (Product # PA5-60151).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RC3H2 in human cell line HeLa shows positivity in cytoplasm. Samples were probed using a RC3H2 Polyclonal Antibody (Product # PA5-60151).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RC3H2 in human cell line HeLa using RC3H2 Polyclonal Antibody (Product # PA5-60151) shows localization to vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RC3H2 in human kidney tissue shows strong cytoplasmic positivity in renal tubules. Samples were probed using a RC3H2 Polyclonal Antibody (Product # PA5-60151).
