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Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- A305-149A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RC3H2/MNAB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human RC3H2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RC3H2 antibody A305-149A (lot A305-149A-1) used for IP at 6 µg per reaction. RC3H2 was also immunoprecipitated by rabbit anti-RC3H2 antibody A305-150A. For blotting immunoprecipitated RC3H2, A305-149A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
