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LearnPA5-28069
antibody from Invitrogen Antibodies
Targeting: TNKS2
ARTD6, PARP-5b, PARP-5c, PARP5B, PARP5C, pART6, TANK2, TNKL
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-28069 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tankyrase 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tankyrase 2 using 30 µg of A) A431 (B) HeLa and C) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Tankyrase 2 polyclonal antibody (Product # PA5-28069) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Tankyrase 2 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Tankyrase 2 Polyclonal Antibody (Product # PA5-28069) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Tankyrase 2 in paraformaldehyde-fixed HeLa cells using a Tankyrase 2 polyclonal antibody (Product # PA5-28069) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
