12948-1-AP

antibody from Proteintech Group

Targeting: IMPDH2

Provider product page for 12948-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [68]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]

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Product number: 12948-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12948-1-AP, RRID:AB_2127351
Product name: IMPDH2 antibody
Antibody type: Polyclonal
Description: KD/KO validated IMPDH2 antibody (Cat. #12948-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IMPDH2 protein structure - 12948-1-AP shown in red.

Submitted references AVN944 Elicits Apoptotic Responses and Impedes Tumorigenic Potential in Ewing's Sarcoma Cells.

Lim H, Lee S, Kim G, Lee EJ, Kim J
International journal of biological sciences 2026;22(2):529-552

IMPDH2 facilitates CD4+ T cell activation through AKT/mTOR pathway by upregulating SRPK1 in myasthenia gravis.

Ren Y, Wu T, Liu Z, Li L, Li Y, Xin G, Peng S, Niu J, Chen B, Cai H, Tian Q, Wang L, Zhang H
Clinical immunology (Orlando, Fla.) 2026 Jan;282:110634

Inhibition of the RAC/PAK Signaling Axis Enhances the Potency of MAPK Cascade Inhibitors Against Uveal Melanoma.

Maslov AA, Trageser NH, Kichina JV, Elamir H, Gardner E, Teaman F, Vishwanath V, Dugas SM, Heid J, Maslov AY, Withers HG, Bianchi-Smiraglia A, Leonova KI, Nikiforov MA, Kandel ES
Biomolecules 2025 Oct 7;15(10)

A Novel Allosteric Inhibitor Targeting IMPDH at Y233 Overcomes Resistance to Tyrosine Kinase Inhibitors in Lymphoma.

Pattabiraman N, Lobello C, Rushmore D, Mologni L, Wasik M, Basappa J
Cancers 2025 Oct 21;17(20)

Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells.

Ball STM, Hennessy MJ, Tan Y, Hoettges KF, Perkins ND, Wilkinson DJ, White MRH, Zheng Y, Turner DA
BMC biology 2025 May 12;23(1):126

An IMPDH2 variant associated with neurodevelopmental disorder disrupts purine biosynthesis and somitogenesis.

O'Neill AG, McCartney ME, Wheeler GM, Patel JH, Sanchez-Ramirez G, Kollman JM, Wills AE
bioRxiv : the preprint server for biology 2025 May 11;

Y12C mutation disrupts IMPDH cytoophidia and alters cancer metabolism.

Chang CC, Peng M, Keppeke GD, Tsai LK, Zhang Z, Pai LM, Sung LY, Liu JL
The FEBS journal 2025 Jul;292(14):3676-3695

Diverse Inhibitors of De Novo Purine Synthesis Promote AICAR-Induced AMPK Activation and Glucose Uptake in L6 Myotubes.

Dolinar K, Miš K, Šopar K, Šutar M, Božič M, Kolar M, Hropot T, Garcia-Roves PM, Chibalin AV, Pirkmajer S
BioFactors (Oxford, England) 2025 Jul-Aug;51(4):e70037

Inosine monophosphate dehydrogenase 2 (IMPDH2) modulates response to therapy and chemo-resistance in triple negative breast cancer.

da Silva Fernandes T, Gillard BM, Dai T, Martin JC, Chaudhry KA, Dugas SM, Fisher AA, Sharma P, Wu R, Attwood KM, Dasgupta S, Takabe K, Rosario SR, Bianchi-Smiraglia A
Scientific reports 2025 Jan 7;15(1):1061

IMPDH2 dephosphorylation under FGFR signaling promotes S-phase progression and tumor growth.

Zhou B, Zhao Q, Hou G, He J, Sha N, Zheng K, Peng H, Wang W, Zhou Y, Chen T, Jiang Y
Cell reports 2025 Jan 28;44(1):115116

An IMPDH2 variant associated with neurodevelopmental disorder disrupts purine biosynthesis and somite organization.

O'Neill AG, McCartney ME, Wheeler GM, Patel JH, Sanchez-Ramirez G, Kollman JM, Wills AE
Proceedings of the National Academy of Sciences of the United States of America 2025 Dec 9;122(49):e2511727122

Impdh2 deficiency suppresses osteoclastogenesis through mitochondrial oxidative phosphorylation and alleviates ovariectomy-induced osteoporosis.

Xu C, Wei Z, Lv L, Dong X, Xia W, Xing J, Liu H, Zhao X, Liu Y, Wang W, Jiang H, Gong Y, Liu C, Xu K, Wang S, Akimoto Y, Hu Z
Biochemical and biophysical research communications 2024 Oct 1;727:150317

Nuclear IMPDH2 controls the DNA damage response by modulating PARP1 activity.

Espinar L, Garcia-Cao M, Schmidt A, Kourtis S, Gañez Zapater A, Aranda-Vallejo C, Ghose R, Garcia-Lopez L, Sheraj I, Pardo-Lorente N, Bantulà M, Pascual-Reguant L, Darai E, Guirola M, Montero J, Sdelci S
Nature communications 2024 Nov 12;15(1):9515

The IMPDH cytoophidium couples metabolism and fetal development in mice.

Peng M, Keppeke GD, Tsai LK, Chang CC, Liu JL, Sung LY
Cellular and molecular life sciences : CMLS 2024 May 8;81(1):210

Lower ratio of IMPDH1 to IMPDH2 sensitizes gliomas to chemotherapy.

Ruan X, Xiong Y, Li X, Yang E, Wang J
Cancer gene therapy 2024 Jul;31(7):1081-1089

Deletion of Impdh2 in adipocyte precursors limits the expansion of white adipose tissue and enhances metabolic health with overnutrition.

Hu J, Zheng S, Hua M, Ding M, Hu Z, Jiang H
Biochemical and biophysical research communications 2024 Jul 5;716:149998

De novo and salvage purine synthesis pathways across tissues and tumors.

Tran DH, Kim D, Kesavan R, Brown H, Dey T, Soflaee MH, Vu HS, Tasdogan A, Guo J, Bezwada D, Al Saad H, Cai F, Solmonson A, Rion H, Chabatya R, Merchant S, Manales NJ, Tcheuyap VT, Mulkey M, Mathews TP, Brugarolas J, Morrison SJ, Zhu H, DeBerardinis RJ, Hoxhaj G
Cell 2024 Jul 11;187(14):3602-3618.e20

Metabolic reprogramming by histone deacetylase inhibition preferentially targets NRF2-activated tumors.

Karagiannis D, Wu W, Li A, Hayashi M, Chen X, Yip M, Mangipudy V, Xu X, Sánchez-Rivera FJ, Soto-Feliciano YM, Ye J, Papagiannakopoulos T, Lu C
Cell reports 2024 Jan 23;43(1):113629

Catalytic isoforms of AMP-activated protein kinase differentially regulate IMPDH activity and photoreceptor neuron function.

Lee TJ, Sasaki Y, Ruzycki PA, Ban N, Lin JB, Wu HT, Santeford A, Apte RS
JCI insight 2024 Jan 16;9(4)

IMPDH2 forms spots at branching sites and distal ends of astrocyte stem processes.

Toyoda S, Handa T, Yong H, Takahashi H, Shiwaku H
Genes to cells : devoted to molecular & cellular mechanisms 2024 Feb;29(2):150-158

Appendage regeneration requires IMPDH2 and creates a sensitized environment for enzyme filament formation.

McCartney ME, Wheeler GM, O'Neill AG, Patel JH, Litt ZR, Calise SJ, Kollman JM, Wills AE
bioRxiv : the preprint server for biology 2024 Aug 21;

Light-sensitive phosphorylation regulates retinal IMPDH1 activity and filament assembly.

Calise SJ, O'Neill AG, Burrell AL, Dickinson MS, Molfino J, Clarke C, Quispe J, Sokolov D, Buey RM, Kollman JM
The Journal of cell biology 2024 Apr 1;223(4)

Effect on cell survival and cytoophidium assembly of the adRP-10-related IMPDH1 missense mutation Asp226Asn.

Keppeke GD, Chang CC, Zhang Z, Liu JL
Frontiers in cell and developmental biology 2023;11:1234592

Light-sensitive phosphorylation regulates enzyme activity and filament assembly of human IMPDH1 retinal splice variants.

Calise SJ, O'Neill AG, Burrell AL, Dickinson MS, Molfino J, Clarke C, Quispe J, Sokolov D, Buey RM, Kollman JM
bioRxiv : the preprint server for biology 2023 Sep 21;

HIF-1α mediates osteoclast-induced disuse osteoporosis via cytoophidia in the femur of mice.

Bie M, Tang Y, Xia Y, Zhang Q, Tian Y, Cheng C, Li X, Qi X, Kang F
Bone 2023 Mar;168:116648

B7-H3 Associates with IMPDH2 and Regulates Cancer Cell Survival.

Alhamad S, Elmasry Y, Uwagboe I, Chekmeneva E, Sands C, Cooper BW, Camuzeaux S, Salam A, Parsons M
Cancers 2023 Jul 7;15(13)

Berberrubine is a novel and selective IMPDH2 inhibitor that impairs the growth of colorectal cancer.

He X, Cui J, Ma H, Abuduaini N, Huang Y, Tang L, Wang W, Zhang Y, Wang Y, Lu W, Feng B, Huang J
Biochemical pharmacology 2023 Dec;218:115868

Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function.

Jiang X, Peng J, Xie Y, Xu Y, Liu Q, Cheng C, Yan P, Xu S, Wang Y, Zhang L, Li H, Li Y, Li B, Han J, Yu D
Cell death and differentiation 2023 Aug;30(8):1931-1942

Identification of IMP Dehydrogenase as a Potential Target for Anti-Mpox Virus Agents.

Hishiki T, Morita T, Akazawa D, Ohashi H, Park ES, Kataoka M, Mifune J, Shionoya K, Tsuchimoto K, Ojima S, Azam AH, Nakajima S, Kawahara M, Yoshikawa T, Shimojima M, Kiga K, Maeda K, Suzuki T, Ebihara H, Takahashi Y, Watashi K
Microbiology spectrum 2023 Aug 17;11(4):e0056623

Microglia-Dependent and Independent Brain Cytoprotective Effects of Mycophenolate Mofetil During Neuronal Damage.

Kleine J, Hohmann U, Hohmann T, Ghadban C, Schmidt M, Laabs S, Alessandri B, Dehghani F
Frontiers in aging neuroscience 2022;14:863598

A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics.

Burclaff J, Bliton RJ, Breau KA, Ok MT, Gomez-Martinez I, Ranek JS, Bhatt AP, Purvis JE, Woosley JT, Magness ST
Cellular and molecular gastroenterology and hepatology 2022;13(5):1554-1589

SARS-CoV-2 Nsp14 protein associates with IMPDH2 and activates NF-κB signaling.

Li TW, Kenney AD, Park JG, Fiches GN, Liu H, Zhou D, Biswas A, Zhao W, Que J, Santoso N, Martinez-Sobrido L, Yount JS, Zhu J
Frontiers in immunology 2022;13:1007089

Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.

Fang YF, Li YL, Li XM, Liu JL
International journal of molecular sciences 2022 Oct 2;23(19)

SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome.

Zaffagni M, Harris JM, Patop IL, Pamudurti NR, Nguyen S, Kadener S
eLife 2022 Mar 16;11

Molecular crowding facilitates bundling of IMPDH polymers and cytoophidium formation.

Chang CC, Peng M, Zhong J, Zhang Z, Keppeke GD, Sung LY, Liu JL
Cellular and molecular life sciences : CMLS 2022 Jul 14;79(8):420

Anti-rods/rings autoantibodies in a patient with pancreatic injury.

Katayama S, Kubota T, Takahashi H, Shiwaku H
Autoimmunity reviews 2022 Jan;21(1):102922

Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer.

Wang S, Chao F, Zhang C, Han D, Xu G, Chen G
Cancer letters 2022 Jan 1;524:109-120

SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome.

Zaffagni M, Harris JM, Patop IL, Pamudurti NR, Nguyen S, Kadener S
bioRxiv : the preprint server for biology 2022 Feb 16;

Coordinated Formation of IMPDH2 Cytoophidium in Mouse Oocytes and Granulosa Cells.

Ni S, Zhang T, Zhou C, Long M, Hou X, You L, Li H, Shi L, Su YQ
Frontiers in cell and developmental biology 2021;9:690536

Gm14230 controls Tbc1d24 cytoophidia and neuronal cellular juvenescence.

Morimune T, Tano A, Tanaka Y, Yukiue H, Yamamoto T, Tooyama I, Maruo Y, Nishimura M, Mori M
PloS one 2021;16(4):e0248517

IMPDH forms the cytoophidium in zebrafish.

Keppeke GD, Chang CC, Antos CL, Peng M, Sung LY, Andrade LEC, Liu JL
Developmental biology 2021 Oct;478:89-101

Nuclear IMPDH Filaments in Human Gliomas.

Ahangari N, Munoz DG, Coulombe J, Gray DA, Engle EC, Cheng L, Woulfe J
Journal of neuropathology and experimental neurology 2021 Oct 26;80(10):944-954

Rewiring of purine metabolism in response to acidosis stress in glioma stem cells.

Xu X, Wang L, Zang Q, Li S, Li L, Wang Z, He J, Qiang B, Han W, Zhang R, Peng X, Abliz Z
Cell death & disease 2021 Mar 15;12(3):277

Shikonin is a novel and selective IMPDH2 inhibitor that target triple-negative breast cancer.

Wang W, Wu Y, Chen S, Liu X, He J, Wang S, Lu W, Tang Y, Huang J
Phytotherapy research : PTR 2021 Jan;35(1):463-476

CTPS and IMPDH form cytoophidia in developmental thymocytes.

Peng M, Chang CC, Liu JL, Sung LY
Experimental cell research 2021 Aug 1;405(1):112662

FANCI Cooperates with IMPDH2 to Promote Lung Adenocarcinoma Tumor Growth via a MEK/ERK/MMPs Pathway.

Zheng P, Li L
OncoTargets and therapy 2020;13:451-463

IMP dehydrogenase rod/ring structures in acral melanomas.

Keppeke GD, Barcelos D, Fernandes M, Comodo AN, Guimarães DP, Cardili L, Carapeto FCL, Andrade LEC, Landman G
Pigment cell & melanoma research 2020 May;33(3):490-497

IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.

Ruan H, Song Z, Cao Q, Ni D, Xu T, Wang K, Bao L, Tong J, Xiao H, Xiao W, Cheng G, Xiong Z, Liang H, Liu D, Wang L, Olivier T, Jane BH, Yang H, Zhang X, Chen K
Molecular therapy : the journal of the American Society of Gene Therapy 2020 May 6;28(5):1299-1313

SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.

Chakraborty A, Lin WC, Lin YT, Huang KJ, Wang PY, Chang IY, Wang HI, Ma KT, Wang CY, Huang XR, Lee YH, Chen BC, Hsieh YJ, Chien KY, Lin TY, Liu JL, Sung LY, Yu JS, Chang YS, Pai LM
Journal of cell science 2020 May 11;133(9)

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Ali ES, Sahu U, Villa E, O'Hara BP, Gao P, Beaudet C, Wood AW, Asara JM, Ben-Sahra I
Molecular cell 2020 Jun 18;78(6):1178-1191.e6

Ribavirin induces widespread accumulation of IMP dehydrogenase into rods/rings structures in multiple major mouse organs.

Keppeke GD, Calise SJ, Chan EKL, Andrade LEC
Antiviral research 2019 Feb;162:130-135

Immune Response-Dependent Assembly of IMP Dehydrogenase Filaments.

Calise SJ, Abboud G, Kasahara H, Morel L, Chan EKL
Frontiers in immunology 2018;9:2789

IMP/GTP balance modulates cytoophidium assembly and IMPDH activity.

Keppeke GD, Chang CC, Peng M, Chen LY, Lin WC, Pai LM, Andrade LEC, Sung LY, Liu JL
Cell division 2018;13:5

Interfilament interaction between IMPDH and CTPS cytoophidia.

Chang CC, Keppeke GD, Sung LY, Liu JL
The FEBS journal 2018 Oct;285(20):3753-3768

Differential capacity of therapeutic drugs to induce Rods/Rings structures in vitro and in vivo and generation of anti-Rods/Rings autoantibodies.

Keppeke GD, Prado MS, Nunes E, Perazzio SF, Rodrigues SH, Ferraz ML, Chan EK, Andrade LE
Clinical immunology (Orlando, Fla.) 2016 Dec;173:149-156

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Calise SJ, Purich DL, Nguyen T, Saleem DA, Krueger C, Yin JD, Chan EK
Journal of cell science 2016 Aug 1;129(15):3042-52

Microinjection of specific anti-IMPDH2 antibodies induces disassembly of cytoplasmic rods/rings that are primarily stationary and stable structures.

Keppeke GD, Andrade LE, Grieshaber SS, Chan EK
Cell & bioscience 2015;5(1):1

Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction.

Keppeke GD, Calise SJ, Chan EK, Andrade LE
Journal of genetics and genomics = Yi chuan xue bao 2015 Jun 20;42(6):287-99

Molecular cell biology and immunobiology of mammalian rod/ring structures.

Carcamo WC, Calise SJ, von Mühlen CA, Satoh M, Chan EK
International review of cell and molecular biology 2014;308:35-74

Ultrastructure of cytoplasmic and nuclear inosine-5'-monophosphate dehydrogenase 2 "rods and rings" inclusions.

Juda P, Smigová J, Kováčik L, Bártová E, Raška I
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2014 Oct;62(10):739-50

Temporal evolution of human autoantibody response to cytoplasmic rods and rings structure during anti-HCV therapy with ribavirin and interferon-α.

Keppeke GD, Satoh M, Ferraz ML, Chan EK, Andrade LE
Immunologic research 2014 Oct;60(1):38-49

Glutamine deprivation initiates reversible assembly of mammalian rods and rings.

Calise SJ, Carcamo WC, Krueger C, Yin JD, Purich DL, Chan EK
Cellular and molecular life sciences : CMLS 2014 Aug;71(15):2963-73

A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions.

Kováčik L, Kereïche S, Kerïeche S, Höög JL, Jůda P, Matula P, Raška I
Journal of structural biology 2014 Apr;186(1):141-52

Hirschsprung-like disease is exacerbated by reduced de novo GMP synthesis.

Lake JI, Tusheva OA, Graham BL, Heuckeroth RO
The Journal of clinical investigation 2013 Nov;123(11):4875-87

Cytoplasmic rods and rings autoantibodies developed during pegylated interferon and ribavirin therapy in patients with chronic hepatitis C.

Covini G, Carcamo WC, Bredi E, von Mühlen CA, Colombo M, Chan EK
Antiviral therapy 2012;17(5):805-11

Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells.

Carcamo WC, Satoh M, Kasahara H, Terada N, Hamazaki T, Chan JY, Yao B, Tamayo S, Covini G, von Mühlen CA, Chan EK
PloS one 2011;6(12):e29690

Identification of IMPDH2 as a tumor-associated antigen in colorectal cancer using immunoproteomics analysis.

He Y, Mou Z, Li W, Liu B, Fu T, Zhao S, Xiang D, Wu Y
International journal of colorectal disease 2009 Nov;24(11):1271-9

Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms.

Gunter JH, Thomas EC, Lengefeld N, Kruger SJ, Worton L, Gardiner EM, Jones A, Barnett NL, Whitehead JP
The international journal of biochemistry & cell biology 2008;40(9):1716-28

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human heart tissue were subjected to SDS PAGE followed by western blot with 12948-1-AP(IMPDH2 antibody) at dilution of 1:400
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using IMPDH2 antibody 12948-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-IMPDH2 (IP:12948-1-AP, 3ug; Detection:12948-1-AP 1:800) with K-562 cells lysate 4000ug.
Sample type: cell line