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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- RQ7329 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Inosine-5'-monophosphate dehydrogenase 2 Antibody / IMPDH2
- Antibody type
- Polyclonal
- Description
- This highly specific Inosine-5'-monophosphate dehydrogenase 2 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Inosine-5'-monophosphate dehydrogenase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) K562, 2) Daudi, 3) U-251 and 4) U937 cell lysate with Inosine-5'-monophosphate dehydrogenase 2 antibody. Predicted molecular weight: ~56 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human T-47D cells with Inosine-5'-monophosphate dehydrogenase 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human ovarian cancer tissue with Inosine-5'-monophosphate dehydrogenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer tissue with Inosine-5'-monophosphate dehydrogenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human cervical cancer tissue with Inosine-5'-monophosphate dehydrogenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human esophageal squamous carcinoma tissue with Inosine-5'-monophosphate dehydrogenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-2 OS cells with Inosine-5'-monophosphate dehydrogenase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Inosine-5'-monophosphate dehydrogenase 2 antibody.
