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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- HPA001400 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA001400, RRID:AB_1079139
- Product name
- Anti-IMPDH2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human IMPDH2, Gene description: IMP (inosine 5'-monophosphate) dehydrogenase 2, Validated applications: IHC, WB, ICC, Uniprot ID: P12268, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Regulation of local GTP availability controls RAC1 activity and cell invasion
Internally ratiometric fluorescent sensors for evaluation of intracellular GTP levels and distribution
Myc-dependent purine biosynthesis affects nucleolar stress and therapy response in prostate cancer
A purine nucleotide biosynthesis enzyme guanosine monophosphate reductase is a suppressor of melanoma invasion.
Antiproliferative effects of AVN944, a novel inosine 5‐monophosphate dehydrogenase inhibitor, in prostate cancer cells
Direct role of nucleotide metabolism in C-MYC-dependent proliferation of melanoma cells.
Bianchi-Smiraglia A, Wolff D, Marston D, Deng Z, Han Z, Moparthy S, Wombacher R, Mussell A, Shen S, Chen J, Yun D, O’Brien Cox A, Furdui C, Hurley E, Feltri M, Qu J, Hollis T, Kengne J, Fongang B, Sousa R, Kandel M, Kandel E, Hahn K, Nikiforov M
Nature Communications 2021;12(1)
Nature Communications 2021;12(1)
Internally ratiometric fluorescent sensors for evaluation of intracellular GTP levels and distribution
Bianchi-Smiraglia A, Rana M, Foley C, Paul L, Lipchick B, Moparthy S, Moparthy K, Fink E, Bagati A, Hurley E, Affronti H, Bakin A, Kandel E, Smiraglia D, Feltri M, Sousa R, Nikiforov M
Nature Methods 2017;14(10):1003-1009
Nature Methods 2017;14(10):1003-1009
Myc-dependent purine biosynthesis affects nucleolar stress and therapy response in prostate cancer
Barfeld S, Fazli L, Persson M, Marjavaara L, Urbanucci A, Kaukoniemi K, Rennie P, Ceder Y, Chabes A, Visakorpi T, Mills I
Oncotarget 2015;6(14):12587-12602
Oncotarget 2015;6(14):12587-12602
A purine nucleotide biosynthesis enzyme guanosine monophosphate reductase is a suppressor of melanoma invasion.
Wawrzyniak JA, Bianchi-Smiraglia A, Bshara W, Mannava S, Ackroyd J, Bagati A, Omilian AR, Im M, Fedtsova N, Miecznikowski JC, Moparthy KC, Zucker SN, Zhu Q, Kozlova NI, Berman AE, Hoek KS, Gudkov AV, Shewach DS, Morrison CD, Nikiforov MA
Cell reports 2013 Oct 31;5(2):493-507
Cell reports 2013 Oct 31;5(2):493-507
Antiproliferative effects of AVN944, a novel inosine 5‐monophosphate dehydrogenase inhibitor, in prostate cancer cells
Floryk D, Thompson T
International Journal of Cancer 2008;123(10):2294-2302
International Journal of Cancer 2008;123(10):2294-2302
Direct role of nucleotide metabolism in C-MYC-dependent proliferation of melanoma cells.
Mannava S, Grachtchouk V, Wheeler LJ, Im M, Zhuang D, Slavina EG, Mathews CK, Shewach DS, Nikiforov MA
Cell cycle (Georgetown, Tex.) 2008 Aug;7(15):2392-400
Cell cycle (Georgetown, Tex.) 2008 Aug;7(15):2392-400
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-IMPDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 shows localization to cytosol & rods & rings.
- Sample type
- Human
