22229-1-AP

antibody from Invitrogen Antibodies

Targeting: TIMM50 TIM50, TIM50L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

22229-1-AP

Provider

Invitrogen Antibodies

Product name

TIMM50 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GNNPVDENG AKIPDEFDND PILVQQLRRT YKYFKDYRQM IIEPTSPCLL PDPLQEPYYQ PPYTLVLELT GVLLHPEWSL ATGWRFKKRP GIETLFQQLA PLYEIVIFTS ETGMTAFPLI DSVDPHGFIS YRLFRDATRY MDGHHVKDIS CLNRDPARVV VVDCKKEAFR LQPYNGVALR PWDGNSDDRV LLDLSAFLKT IALNGVEDVR TVLEHYALED DPLAAFKQRQ SRLEQEEQQR LAELSKSNKQ NLFLGSLTSR LWPRSKQP (190-456 aa encoded by BC121147)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 22229-1-AP (TIMM50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 22229-1-AP (TIMM50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 22229-1-AP (TIMM50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat kidney tissue were subjected to SDS PAGE followed by western blot with 22229-1-AP (TIMM50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 22229-1-AP (TIMM50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney slide using 22229-1-AP (TIMM50 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver slide using 22229-1-AP (TIMM50 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney slide using 22229-1-AP ( TIMM50 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver slide using 22229-1-AP ( TIMM50 Antibody) at dilution of 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TIMM50 (IP:22229-1-AP, 4ug; Detection:22229-1-AP 1:1000) with HeLa cells lysate 3200ug.