MA5-48188

antibody from Invitrogen Antibodies

Targeting: RHD CD240D, DIIIc, RH, Rh30a, Rh4, RhII, RhPI

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Recommended by provider)

Antibody Data

Product number

MA5-48188

Provider

Invitrogen Antibodies

Product name

RHD Chimeric Recombinant Rabbit Monoclonal Antibody (D7C2)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

D7C2

Vial size

200 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of RHD in K562 cells using RHD Chimeric Monoclonal Antibody (Product # MA5-48188). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with RHD Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom RHD Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was a Mouse IgG1 Isotype Control Antibody (Product # MA5-47823) followed by staining with Alexa Fluor 488 secondary antibody.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of RHD in K562 cells using RHD Chimeric Monoclonal Antibody (Product # MA5-48188). Cells were fixed with paraformaldehyde and stained with Rabbit IgG Isotype Control (Product # MA5-47825) (black line) or RHD Chimeric Monoclonal Antibody (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AF488 antibody at a dilution of 1:1,000 and cells analyzed using a flow-cytometer.