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- Antibody Data
- Antigen structure
- References [1]
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- Immunocytochemistry [3]
- Immunohistochemistry [2]
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- Product number
- PA5-26336 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.48 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Comparison of four DLL3 antibodies performance in high grade neuroendocrine lung tumor samples and cell cultures.
Brcic L, Kuchler C, Eidenhammer S, Pabst D, Quehenberger F, Gazdar AF, Popper H
Diagnostic pathology 2019 May 20;14(1):47
Diagnostic pathology 2019 May 20;14(1):47
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 293 cells using a DLL3 polyclonal antibody (Product # PA5-26336) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DLL3 in U-2 OS (human osteosarcoma cell line) cells. Samples were incubated with DLL3 polyclonal antibody (Product # PA5-26336) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DLL3 in U-2 OS (human osteosarcoma cell line) cells. Samples were incubated with DLL3 polyclonal antibody (Product # PA5-26336) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DLL3 in paraffin-embedded human brain tissue. Samples were incubated with DLL3 polyclonal antibody (Product # PA5-26336) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DLL3 in paraffin-embedded Human testis tissue. Samples were incubated with DLL3 polyclonal antibody (Product # PA5-26336) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9.0).
