PA5-87853

antibody from Invitrogen Antibodies

Targeting: TMEM74 FLJ30668, NET36

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87853

Provider

Invitrogen Antibodies

Product name

TMEM74 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MELHYLAKKS NQADLCDARD WSSRGLPGDQ ADTAATRAAL CCQKQCASTP RATEMEGSKL SSSPASPSSS LQNSTLQPDA FPPGLLHSGN NQITAERKVC NCCSQELETS FTYVDKNINL EQRNRSSPSA KGHNHPGELG WENPNEWSQE AAISLISEEE DDTSSEATSS GKSIDYGFIS; Positive Samples: DU145; Cellular Location: Cytoplasmic vesicle, Lysosome membrane, Multi-pass membrane protein, autophagosome membrane

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.01 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of DU145 cells, using TMEM74 Polyclonal antibody (Product # PA5-87853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.