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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-24251 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Galectin 10 Monoclonal Antibody (561603)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2, -3, -4, -7, -8, or -9/Ecalectin is observed. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 561603
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human eosinophils (enriched, approximately 60%). PVDF Membrane was probed with 1 µg/mL of human Galectin-10 Monoclonal Antibody (Product # MA5-24251) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Galectin-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Galectin 10 in human eosinophils (enriched, approximately 60%). Samples were incubated in Galectin 10 monoclonal antibody (Product # MA5-24251) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Galectin‚10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Galectin 10 in immersion fixed HL‚60 human acute promyelocytic leukemia cell line. Samples were incubated in Galectin 10 monoclonal antibody (Product # MA5-24251) using a dilution of 8 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of human peripheral blood lymphocytes were stained with Human Galectin-10 Monoclonal Antibody (Product # MA5-24251) followed by FITC-conjugated Anti-Mouse IgG Secondary Antibody and Human IL-2Rα APC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Galectin 10 in Human peripheral blood lymphocytes. Samples were incubated in Galectin 10 monoclonal antibody (Product # MA5-24251) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody and Human IL‚2 Rα APC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining.
