14119-1-AP

antibody from Proteintech Group

Targeting: MARCHF8 c-MIR, CMIR, MARCH-VIII, MARCH8, MIR, RNF178

Provider product page for 14119-1-AP

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Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [36]
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Validations
Western blot [1]

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Product number: 14119-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14119-1-AP, RRID:AB_2140168
Product name: MARCH8 antibody
Antibody type: Polyclonal
Description: KD/KO validated MARCH8 antibody (Cat. #14119-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MARCHF8 protein structure - 14119-1-AP shown in red.

Submitted references The E3 ubiquitin ligase MARCHF8 restricts HSV-1 infection by inhibiting replication of the viral genome.

Xia Y, Nigos LR, Villalón-Letelier F, Tessema MB, Brooks AG, Bartok E, Behrendt R, Londrigan SL, Reading PC, Farrukee R
The Journal of biological chemistry 2025 Sep;301(9):110567

WTAP Mediated m6A Modification Stabilizes PDIA3P1 and Promotes Tumor Progression Driven by Histone Lactylation in Esophageal Squamous Cell Carcinoma.

Huang T, You Q, Liu J, Shen X, Huang D, Tao X, He Z, Wu C, Xi X, Yu S, Liu F, Wu Z, Mao W, Zhu S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(33):e06529

Transferrin receptor controls both autophagosome formation and closure via phosphatidylinositol 3-phosphate synthesis.

Puri C, Park SJ, Wrobel L, Rubinsztein DC
Developmental cell 2025 Oct 20;60(20):2715-2729.e8

PABPC4 Inhibits SADS-CoV Replication by Degrading the Nucleocapsid Protein Through Selective Autophagy.

Zhao C, Qin Y, Huang H, Chen W, Hu Y, Zhang X, Li Y, Lan T, Sun W
Veterinary sciences 2025 Mar 10;12(3)

miR-34a-5p/MARCHF8/ADAM10 axis in the regulation of vascular endothelial cell dysfunction and senescence.

Qian Z, Huang Y, Yang N, Fang Z, Zhang Y, Huang Y, Luo M, Ji T, Chen Z, Gao S, Li Y, Yan J, Jiang D, Ruan L, Liu A, Zhang C, Zhang L
Mechanisms of ageing and development 2025 Jun;225:112060

Midnolin inhibits coronavirus proliferation by degrading viral proteins.

Wang Y, Tong W, Qin W, Yang X, Yu H, Zheng H, Zhang W, Tong G, Wang C, Kong N, Shan T
Journal of virology 2025 Jun 17;99(6):e0036625

Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1.

Huang XR, Ye L, An N, Wu CY, Wu HL, Li HY, Huang YH, Ye QR, Liu MD, Yang LW, Liu JX, Tang JX, Pan QJ, Wang P, Sun L, Xia Y, Lan HY, Yang C, Liu HF
Autophagy 2025 Jan;21(1):120-140

MARCH8-mediated ubiquitination regulates expression of the antiviral protein IFITM3.

Wei L, Zhao F, Liu X, Mei S, Huang Y, Xie Y, Hu Y, Wang L, Wang L, Gao Z, Chen C, Shi Y, He Y, Wang J, Xue T, Xu F, Fang J, Guo F
The Journal of biological chemistry 2025 Dec;301(12):110879

A pan-cancer analysis of MARCH8: molecular characteristics, clinical relevance, and immuno-oncology features.

Quan Z, Fan S, Zheng H, Ning Y, Yang Y
Cancer biology & therapy 2025 Dec;26(1):2458773

BST-2 Promotes N Protein Degradation and Inhibits Viral Replication Through the MARCHF8/NDP52 Autophagy Pathway.

Zhao C, Qin Y, Huang H, Li Y, Zhang X, Zhou L, Xie L, Zhou Y, Hu Y, Chen W, Lan T, Sun WC
Microorganisms 2025 Aug 9;13(8)

NPR1 promotes cisplatin resistance by inhibiting PARL-mediated mitophagy-dependent ferroptosis in gastric cancer.

Wu C, Wang S, Huang T, Xi X, Xu L, Wang J, Hou Y, Xia Y, Xu L, Wang L, Huang X
Cell biology and toxicology 2024 Oct 30;40(1):93

NLRP12 inhibits PRRSV-2 replication by promoting GP2a degradation via MARCH8.

Jing H, Song Y, Duan E, Liu J, Ke W, Tao R, Lv Y, Zhao P, Dong W, Li X, Guo Y, Li H
Veterinary microbiology 2024 Nov;298:110271

MARCH8 Mediates K27-Linked Polyubiquitination of IL-7 Receptor α to Negatively Regulate IL-7-Triggered T Cell Homeostasis.

Gao D, Yi XM, Feng L, Li S, Shu HB
Journal of immunology (Baltimore, Md. : 1950) 2024 Nov 15;213(10):1467-1478

O-GlcNAcylation regulates the stability of transferrin receptor (TFRC) to control the ferroptosis in hepatocellular carcinoma cells.

Zhou X, Wang Y, Li X, Zhou J, Yang W, Wang X, Jiao S, Zuo W, You Z, Ying W, Wu C, Bao J
Redox biology 2024 Jul;73:103182

The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer.

Khalil MI, Yang C, Vu L, Chadha S, Nabors H, James CD, Morgan IM, Pyeon D
Journal of virology 2024 Feb 20;98(2):e0172623

MARCH8 Restricts RSV Replication by Promoting Cellular Apoptosis Through Ubiquitin-Mediated Proteolysis of Viral SH Protein.

Okura T, Takahashi T, Kameya T, Mizukoshi F, Nakai Y, Kakizaki M, Nishi M, Otsuki N, Kimura H, Miyakawa K, Shirato K, Kamitani W, Ryo A
Viruses 2024 Dec 18;16(12)

The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer.

Khalil MI, Yang C, Vu L, Chadha S, Nabors H, James CD, Morgan IM, Pyeon D
bioRxiv : the preprint server for biology 2023 Nov 4;

MARCH8 downregulation modulates profibrotic responses including myofibroblast differentiation.

Guo X, Adeyanju O, Olajuyin AM, Mandlem V, Sunil C, Adewumi J, Huang S, Tucker TA, Idell S, Qian G
American journal of physiology. Cell physiology 2023 Nov 1;325(5):C1190-C1200

N protein of PEDV plays chess game with host proteins by selective autophagy.

Zhai X, Kong N, Zhang Y, Song Y, Qin W, Yang X, Ye C, Ye M, Tong W, Liu C, Zheng H, Yu H, Zhang W, Yang X, Zhang G, Tong G, Shan T
Autophagy 2023 Aug;19(8):2338-2352

TARDBP Inhibits Porcine Epidemic Diarrhea Virus Replication through Degrading Viral Nucleocapsid Protein and Activating Type I Interferon Signaling.

Dong S, Kong N, Zhang Y, Li Y, Sun D, Qin W, Zhai H, Zhai X, Yang X, Ye C, Ye M, Liu C, Yu L, Zheng H, Tong W, Yu H, Zhang W, Tong G, Shan T
Journal of virology 2022 May 25;96(10):e0007022

FUBP3 Degrades the Porcine Epidemic Diarrhea Virus Nucleocapsid Protein and Induces the Production of Type I Interferon.

Dong S, Kong N, Wang C, Li Y, Sun D, Qin W, Zhai H, Zhai X, Yang X, Ye C, Ye M, Liu C, Yu L, Zheng H, Tong W, Yu H, Zhang W, Tong G, Shan T
Journal of virology 2022 Jul 13;96(13):e0061822

MARCH3 negatively regulates IL-3-triggered inflammatory response by mediating K48-linked polyubiquitination and degradation of IL-3Rα.

Feng L, Li C, Zeng LW, Gao D, Sun YH, Zhong L, Lin H, Shu HB, Li S
Signal transduction and targeted therapy 2022 Jan 24;7(1):21

2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8.

Sun D, Kong N, Dong S, Chen X, Qin W, Wang H, Jiao Y, Zhai H, Li L, Gao F, Yu L, Zheng H, Tong W, Yu H, Zhang W, Tong G, Shan T
Autophagy 2022 Aug;18(8):1969-1981

The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector Transduction.

Xu F, Liu X, Zhang D, Zhao F, Fan Z, Hu S, Mei S, Huang Y, Sun H, Wei L, Guo L, Wang J, Cen S, Liang C, Guo F
Human gene therapy 2021 Sep;32(17-18):936-948

EGR1 Suppresses Porcine Epidemic Diarrhea Virus Replication by Regulating IRAV To Degrade Viral Nucleocapsid Protein.

Wang H, Kong N, Jiao Y, Dong S, Sun D, Chen X, Zheng H, Tong W, Yu H, Yu L, Zhang W, Tong G, Shan T
Journal of virology 2021 Sep 9;95(19):e0064521

Membrane-Associated RING-CH 8 Functions as a Novel PD-L1 E3 Ligase to Mediate PD-L1 Degradation Induced by EGFR Inhibitors.

Qian G, Guo J, Vallega KA, Hu C, Chen Z, Deng Y, Wang Q, Fan S, Ramalingam SS, Owonikoko TK, Wei W, Sun SY
Molecular cancer research : MCR 2021 Oct;19(10):1622-1634

PABPC4 Broadly Inhibits Coronavirus Replication by Degrading Nucleocapsid Protein through Selective Autophagy.

Jiao Y, Kong N, Wang H, Sun D, Dong S, Chen X, Zheng H, Tong W, Yu H, Yu L, Huang Y, Wang H, Sui B, Zhao L, Liao Y, Zhang W, Tong G, Shan T
Microbiology spectrum 2021 Oct 31;9(2):e0090821

MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells.

Chen W, Patel D, Jia Y, Yu Z, Liu X, Shi H, Liu H
Cancers 2021 May 22;13(11)

Elucidating the Antiviral Mechanism of Different MARCH Factors.

Umthong S, Lynch B, Timilsina U, Waxman B, Ivey EB, Stavrou S
mBio 2021 Mar 2;12(2)

MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.

Liu X, Xu F, Ren L, Zhao F, Huang Y, Wei L, Wang Y, Wang C, Fan Z, Mei S, Song J, Zhao Z, Cen S, Liang C, Wang J, Guo F
Nature communications 2021 Jul 20;12(1):4427

MARCH8 Inhibits Ebola Virus Glycoprotein, Human Immunodeficiency Virus Type 1 Envelope Glycoprotein, and Avian Influenza Virus H5N1 Hemagglutinin Maturation.

Yu C, Li S, Zhang X, Khan I, Ahmad I, Zhou Y, Li S, Shi J, Wang Y, Zheng YH
mBio 2020 Sep 15;11(5)

BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.

Kong N, Shan T, Wang H, Jiao Y, Zuo Y, Li L, Tong W, Yu L, Jiang Y, Zhou Y, Li G, Gao F, Yu H, Zheng H, Tong G
Autophagy 2020 Oct;16(10):1737-1752

Tetherin Suppresses Type I Interferon Signaling by Targeting MAVS for NDP52-Mediated Selective Autophagic Degradation in Human Cells.

Jin S, Tian S, Luo M, Xie W, Liu T, Duan T, Wu Y, Cui J
Molecular cell 2017 Oct 19;68(2):308-322.e4

JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells.

Wang Q, Chen Q, Zhu L, Chen M, Xu W, Panday S, Wang Z, Li A, Røe OD, Chen R, Wang S, Zhang R, Zhou J
Oncogenesis 2017 Jul 3;6(7):e353

Cell adhesion in zebrafish embryos is modulated by March 8.

Kim MH, Rebbert ML, Ro H, Won M, Dawid IB
PloS one 2014;9(4):e94873

The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation.

Chen R, Li M, Zhang Y, Zhou Q, Shu HB
Proceedings of the National Academy of Sciences of the United States of America 2012 Aug 28;109(35):14128-33

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The MARCH8 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MARCH8. This antibody recognizes human, mouse antigen. The MARCH8 antibody has been validated for the following applications: ELISA, WB, IP analysis.
Appendix: 14119-1-AP.pdf

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