PA5-105874

antibody from Invitrogen Antibodies

Targeting: DYRK1B MIRK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-105874

Provider

Invitrogen Antibodies

Product name

Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of DYRK1A/B only when phosphorylated at Tyr321/273.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) in EGF treated K562 cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) polyclonal antibody (Product # PA5-105874).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) in human liver cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) polyclonal antibody (Product # PA5-105874) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) in human normal tissues adjacent to pancreatic cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) polyclonal antibody (Product # PA5-105874) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) polyclonal antibody (Product # PA5-105874) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) in rat lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) polyclonal antibody (Product # PA5-105874) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) in human liver cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-DYRK1A/DYRK1B (Tyr321, Tyr273) polyclonal antibody (Product # PA5-105874) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.