Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- LS-C290549 - Provider product page
- Provider
- LSBio
- Product name
- KIAA0430 Antibody (aa1625-1675) LS-C290549
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human and Mouse Limkain b1 by Western Blot. Samples: Whole cell lysate from Jurkat (15 and 50 ug), HeLa (50 ug), 293T (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-Limkain b1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Limkain b1 by Western Blot. Samples: Whole cell lysate from Jurkat (15 and 50 ug), HeLa (50 ug), 293T (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-Limkain b1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human Limkain b1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Limkain b1 antibody used for IP at 6 ug/mg lysate. Limkain b1 was also immunoprecipitated by rabbit anti-Limkain b1 antibody BL14068. For blotting immunoprecipitated Limkain b1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.