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- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 16726-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16726-1-AP, RRID:AB_2247351
- Product name
- GCSH antibody
- Antibody type
- Polyclonal
- Description
- GCSH antibody (Cat. #16726-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Clinical features and molecular landscape of cuproptosis signature-related molecular subtype in gastric cancer.
FDX1 regulates cellular protein lipoylation through direct binding to LIAS.
FDX1 regulates cellular protein lipoylation through direct binding to LIAS.
Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin.
Copper induces cell death by targeting lipoylated TCA cycle proteins.
Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.
Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton.
Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats.
Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.
GCSH antisense regulation determines breast cancer cells' viability.
Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.
Amino acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.
Chong W, Ren H, Chen H, Xu K, Zhu X, Liu Y, Sang Y, Li H, Liu J, Ye C, Shang L, Jing C, Li L
iMeta 2024 Jun;3(3):e190
iMeta 2024 Jun;3(3):e190
FDX1 regulates cellular protein lipoylation through direct binding to LIAS.
Dreishpoon MB, Bick NR, Petrova B, Warui DM, Cameron A, Booker SJ, Kanarek N, Golub TR, Tsvetkov P
The Journal of biological chemistry 2023 Sep;299(9):105046
The Journal of biological chemistry 2023 Sep;299(9):105046
FDX1 regulates cellular protein lipoylation through direct binding to LIAS.
Dreishpoon MB, Bick NR, Petrova B, Warui DM, Cameron A, Booker SJ, Kanarek N, Golub TR, Tsvetkov P
bioRxiv : the preprint server for biology 2023 Feb 4;
bioRxiv : the preprint server for biology 2023 Feb 4;
Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin.
Head PE, Myung S, Chen Y, Schneller JL, Wang C, Duncan N, Hoffman P, Chang D, Gebremariam A, Gucek M, Manoli I, Venditti CP
Science translational medicine 2022 May 25;14(646):eabn4772
Science translational medicine 2022 May 25;14(646):eabn4772
Copper induces cell death by targeting lipoylated TCA cycle proteins.
Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub TR
Science (New York, N.Y.) 2022 Mar 18;375(6586):1254-1261
Science (New York, N.Y.) 2022 Mar 18;375(6586):1254-1261
Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.
Yang J, Li Y, Gao W, Feng Y, He X, Ni H, Song X, Fan J
Experimental eye research 2022 Jun;219:109052
Experimental eye research 2022 Jun;219:109052
Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
Xia J, Zhang J, Wu X, Du W, Zhu Y, Liu X, Liu Z, Meng B, Guo J, Yang Q, Wang Y, Wang Q, Feng X, Xie G, Shen Y, He Y, Xiang J, Wu M, An G, Qiu L, Jia W, Zhou W
Nature communications 2022 Jul 11;13(1):4007
Nature communications 2022 Jul 11;13(1):4007
Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton.
Adamus A, Ali I, Vasileiadis V, Al-Hileh L, Lisec J, Frank M, Seitz G, Engel N
BMC complementary medicine and therapies 2021 May 4;21(1):136
BMC complementary medicine and therapies 2021 May 4;21(1):136
Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats.
Simmons RM, McKnight SM, Edwards AK, Wu G, Satterfield MC
Amino acids 2020 Oct;52(10):1413-1423
Amino acids 2020 Oct;52(10):1413-1423
Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.
Tian S, Feng J, Cao Y, Shen S, Cai Y, Yang D, Yan R, Wang L, Zhang H, Zhong X, Gao P
Life science alliance 2019 Oct;2(5)
Life science alliance 2019 Oct;2(5)
GCSH antisense regulation determines breast cancer cells' viability.
Adamus A, Müller P, Nissen B, Kasten A, Timm S, Bauwe H, Seitz G, Engel N
Scientific reports 2018 Oct 18;8(1):15399
Scientific reports 2018 Oct 18;8(1):15399
Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.
Crooks DR, Maio N, Lane AN, Jarnik M, Higashi RM, Haller RG, Yang Y, Fan TW, Linehan WM, Rouault TA
The Journal of biological chemistry 2018 May 25;293(21):8297-8311
The Journal of biological chemistry 2018 May 25;293(21):8297-8311
Amino acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.
Johnson MA, Vidoni S, Durigon R, Pearce SF, Rorbach J, He J, Brea-Calvo G, Minczuk M, Reyes A, Holt IJ, Spinazzola A
PloS one 2014;9(4):e93597
PloS one 2014;9(4):e93597
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 16726-1-AP(GCSH antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using GCSH antibody 16726-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 16726-1-AP(GCSH antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 16726-1-AP(GCSH antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
