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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [3]
- Immunohistochemistry [6]
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Validation data
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- Product number
- LS-C114648 - Provider product page
- Provider
- LSBio
- Product name
- NEK6 Antibody (clone OTI2H7) LS-C114648
- Antibody type
- Monoclonal
- Description
- Protein A/G purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2H7
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation(IP) of NEK6 by using monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-NEK6 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of NEK6 by using monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-NEK6 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of NEK6 by using monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-NEK6 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of kidney tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Ovary tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded endometrium tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
