Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-47865 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47865, RRID:AB_10010849
- Product name
- Mouse Monoclonal NEK6 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified. This antibody is specific for Homo sapiens NIMA (never in mitosis gene a)-related kinase 6 (NEK6), transcript variant 2.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.75 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEK6 Antibody (5D7) [NBP1-47865] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEK6 Antibody (OTI5D7) [NBP1-47865] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK6 Antibody (5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NEK6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NEK6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NEK6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NEK6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NEK6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NEK6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NEK6 Antibody (OTI5D7) [NBP1-47865] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK6 antibody, and then analyzed by flow cytometry.