H00009533-M05

antibody from Abnova Corporation

Targeting: POLR1C AC40, RPA39, RPA40, RPA5, RPAC1, RPC40

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009533-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009533-M05, RRID:AB_464069

Product name

POLR1C monoclonal antibody (M05), clone M1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant POLR1C.

Antigen sequence

MAASQAVEEMRSRVVLGEFGVRNVHTTDFPGNYSG
YDDAWDQDRFEKNFRVDVVHMDENSLEFDMVGIDA
AIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEI
LAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQF
RLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTW
IPLGNQADLFPEGTIRPVHDDILIAQLRPGQEIDL
LMHCVKGIGKDHAKFSPVATASYRLLPDITLLEPV
EGEAAEELSRCFSPGVIEVQEVQGKKVARVANPRL
DTFSREIFRNEKLKKVVRLARVRDHYIFSVESTGV
LPPDVLVSEAIKVLMGKCRRFLDELDAVQMD


Isotype

IgG

Antibody clone number

M1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

POLR1C monoclonal antibody (M05), clone M1 Western Blot analysis of POLR1C expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged POLR1C is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to POLR1C on formalin-fixed paraffin-embedded human pancreatic cancer. [antibody concentration 0.6 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol