Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- GTX111657 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111657, RRID:AB_1950172
- Product name
- EFEMP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Metformin Treatment Suppresses Melanoma Cell Growth and Motility Through Modulation of microRNA Expression.
Tseng HW, Li SC, Tsai KW
Cancers 2019 Feb 11;11(2)
Cancers 2019 Feb 11;11(2)
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EFEMP1 antibody detects EFEMP1 protein by Western blot analysis.A. 50 µg rat liver lysate/extract 10 % SDS-PAGEEFEMP1 antibody (GTX111657) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 10% SDS PAGE GTX111657 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EFEMP1 antibody detects EFEMP1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g HepG2 whole cell lysate/extract10% SDS-PAGEEFEMP1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EFEMP1 antibody detects EFEMP1 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 10% SDS-PAGE, and blotted with EFEMP1 antibody (GTX111657) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EFEMP1 antibody detects EFEMP1 protein by western blot analysis. Rat tissue extracts (50 ?g) was separated by 10% SDS-PAGE, and blotted with EFEMP1 antibody (GTX111657) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody (GTX111657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Fibulin 3(GTX111657) antibody at 1:500 dilution.