Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- OAAB05684 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- EFEMP1 antibody - N-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of EFEMP1 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). EFEMP1 (arrow) was detected using the purified Pab.
- Sample type
- mouse lung tissue lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EFEMP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Sample type
- human lung carcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- EFEMP1 Antibody (N-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- NCI-H460 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
