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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-26104 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fibulin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Fluoro-edenite induces fibulin-3 overexpression in non-malignant human mesothelial cells.
Rapisarda V, Salemi R, Marconi A, Loreto C, Graziano AC, Cardile V, Basile MS, Candido S, Falzone L, Spandidos DA, Fenga C, Libra M
Oncology letters 2016 Nov;12(5):3363-3367
Oncology letters 2016 Nov;12(5):3363-3367
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Fibulin 3 in formalin-fixed and paraffin-embedded human lung carcinoma. Samples were incubated with Fibulin 3 polyclonal antibody (Product # PA5-26104) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of NCI-H460 cells using an EFEMP1 polyclonal antibody (Product # PA5-26104) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Fibulin 3 in NCI-H460 cells (bottom histogram). Samples were incubated with Fibulin 3 polyclonal antibody (Product # PA5-26104) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
