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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP51374PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51374PU-N, RRID:AB_11144608
- Product name
- anti Fibulin-3 (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 126-156aa) of human Fibulin-3
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Fibulin-3 (arrow) in mouse lung tissue lysates (35ug/lane) using Fibulin-3Â antibody Cat.-No. AP51374PU-NÂ (N-term).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human lung carcinoma (Formalin-fixed, Paraffin-embedded) using Fibulin-3 antibody Cat.-No. AP51374PU-N (N-term), followed by peroxidase-conjugated secondary antibodyand DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram) using Fibulin-3 antibody Cat.-No. AP51374PU-N (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
