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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-49468 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBMX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of host proteins differentially associated with HIV-1 RNA splice variants.
Knoener R, Evans E 3rd, Becker JT, Scalf M, Benner B, Sherer NM, Smith LM
eLife 2021 Feb 25;10
eLife 2021 Feb 25;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of RBMX (green) in human fibroblasts. Cells were stained with a RBMX polyclonal antibody (Product # PA5-49468) at a dilution of 1:25, washed extensively followed by an incubation with anti-rabbit Alexa Fluor 488 (Product # A-21206) at a dilution of 1:500 (green). Nucleoplasm staining is seen in images which were taken at a 63X magnification. Data courtesy of Antibody Data Exchange Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RBMX in paraformaldehyde-fixed, paraffin-embedded human testis tissue sections. Samples were incubated with RBMX polyclonal antibody (Product # PA5-49468) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).
