Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-30988 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMB7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, mouse brain.
- Concentration
- 1 mg/mL
Submitted references Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates.
Kim S, Park SH, Choi WH, Lee MJ
Immune network 2022 Jun;22(3):e28
Immune network 2022 Jun;22(3):e28
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PSMB7 Polyclonal Antibody (Product # PA5-30988). Sample (30 µg of whole cell lysate). Lane A: HepG2. 12% SDS PAGE. PSMB7 Polyclonal Antibody (Product # PA5-30988) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PSMB7 Polyclonal Antibody (Product # PA5-30988). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. PSMB7 Polyclonal Antibody (Product # PA5-30988) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Proteasome 20S beta 7 in paraformaldehyde-fixed HeLa cells using a Proteasome 20S beta 7 polyclonal antibody (Product # PA5-30988) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using Proteasome 20S beta 7 (Product # PA5-30988) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSMB7 Polyclonal Antibody detects Proteasome 20S beta 7 protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PSMB7 Polyclonal Antibody (Product # PA5-30988) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.