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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-29454 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CEP55 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, U87-MG, MCF-7, HeLa. Predicted reactivity: Rat (80%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.85 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CEP55 Polyclonal Antibody (Product # PA5-29454). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 10% SDS PAGE. CEP55 Polyclonal Antibody (Product # PA5-29454) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of differential subcellular localization of CEP55 protein during different phases of mitosis demonstrates antibody specificity. Immunofluorescence analysis using Anti-CEP55 Polyclonal Antibody (Product # PA5-29454), shows its expression at different phases of mitosis in NTERA cells where it is associated with the centrosome throughout the mitotic division. It can be seen at high levels in the condensing chromosomes during Prophase; concentrated near the spindle poles during Metaphase, starts separating in the spindle midzone at Anaphase and localizes in the midbody between two daughter cells at Telophase. The images were captured at 60X magnification.
