Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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Validation data
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- Product number
- 12946-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VRK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MPPKRNEKY KLPIPFPEGK VLDDMEGNQW VLGKKIGSGG FGLIYLAFPT NKPEKDARHV VKVEYQENGP LFSELKFYQR VAKKDCIKKW IERKQLDYLG IPLFYGSGLT EFKGRSYRFM VMERLGIDLQ KISGQNGTFK KSTVLQLGIR MLDVLEYIHE NEYVHGDVKA ANLLLGYKNP DQVYLADYGL SYRYCPNGNH KQYQENPRKG HNGTIEFTSL DAHKGVALSR RSDVEILGYC MLRWLCGKLP WEQNLKDPVA VQTAKTNLLD ELPQSVLKWA PSGSSCCEIA QFLVCAHSLA YDEKPNYQAL KKILNPHGIP LGPLDFSTKG QSINVHTPNS QKVDSQKAAT KQVN (1-353 aa encoded by BC027854)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-VRK2 (IP:12946-1-AP, 4ug; Detection:12946-1-AP 1:500) with K-562 cells lysate 3600ug.