Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Other assay [5]
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- Product number
- 14-9767-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Golgin-97 Monoclonal Antibody (CDF4), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody CDF4 recognizes human Golgin-97. Golgin-97, a GRIP domain protein member that was first identified as a Golgi complex autoantigen associated with Sjogren's syndrome. Golgin-97 is a peripheral membrane protein found on the trans face of the Golgi apparatus. The protein G family member, Arl1, mediates the binding of Golgin-97 to the Golgi membrane. Like all GRIP proteins, Golgin-97 functions in maintaining the integrity of the trans Golgi network. Golgin-97 has also been shown to function as a tethering molecule in the retrograde transport of proteins from the endosomes to the trans Golgi network. During poxvirus infection, Golgin-97 incorporates into virions and plays a role in viral replication and morphogenesis. Applications Reported: This CDF4 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. Applications Tested: This CDF4 antibody has been tested by immunocytochemistry of fixed and permeabilized HeLa cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CDF4
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references The Tumour Suppressor TMEM127 Is a Nedd4-Family E3 Ligase Adaptor Required by Salmonella SteD to Ubiquitinate and Degrade MHC Class II Molecules.
A trans-Golgi network resident protein, golgin-97, accumulates in viral factories and incorporates into virions during poxvirus infection.
Autoantigen Golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-golgi network.
A role for GRIP domain proteins and/or their ligands in structure and function of the trans Golgi network.
Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjögren's syndrome.
Alix E, Godlee C, Cerny O, Blundell S, Tocci R, Matthews S, Liu M, Pruneda JN, Swatek KN, Komander D, Sleap T, Holden DW
Cell host & microbe 2020 Jul 8;28(1):54-68.e7
Cell host & microbe 2020 Jul 8;28(1):54-68.e7
A trans-Golgi network resident protein, golgin-97, accumulates in viral factories and incorporates into virions during poxvirus infection.
Alzhanova D, Hruby DE
Journal of virology 2006 Dec;80(23):11520-7
Journal of virology 2006 Dec;80(23):11520-7
Autoantigen Golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-golgi network.
Lu L, Tai G, Hong W
Molecular biology of the cell 2004 Oct;15(10):4426-43
Molecular biology of the cell 2004 Oct;15(10):4426-43
A role for GRIP domain proteins and/or their ligands in structure and function of the trans Golgi network.
Yoshino A, Bieler BM, Harper DC, Cowan DA, Sutterwala S, Gay DM, Cole NB, McCaffery JM, Marks MS
Journal of cell science 2003 Nov 1;116(Pt 21):4441-54
Journal of cell science 2003 Nov 1;116(Pt 21):4441-54
Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjögren's syndrome.
Griffith KJ, Chan EK, Lung CC, Hamel JC, Guo X, Miyachi K, Fritzler MJ
Arthritis and rheumatism 1997 Sep;40(9):1693-702
Arthritis and rheumatism 1997 Sep;40(9):1693-702
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized HeLa cells stained with 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-Human Golgin-97 Purified (right) followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized HeLa cells stained with 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-Human Golgin-97 Purified (right) followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
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