PA5-20510

antibody from Invitrogen Antibodies

Targeting: UNC93B1 UNC93

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20510

Provider

Invitrogen Antibodies

Product name

UNC93B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is human heart tissue lysate. PA5-20510 can be used with blocking peptide PEP-0630.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity.

Majer O, Liu B, Kreuk LSM, Krogan N, Barton GM
Nature 2019 Nov;575(7782):366-370

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of human heart cells using a Unc93b polyclonal antibody (Product # PA5-20510) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of Unc93b in Human Heart cells with UNC93B1 Polyclonal Antibody (Product # PA5-20510) at 20 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Extended Data Fig. 9: Unc93b1 PKP knock-in mice develop systemic inflammation. ( a ) CRISPR/Cas9 strategy to generate Unc93b1 PKP knock-in mice. Green line indicates the guide sequence. Red bases indicate the edited codons. A representative sequencing trace of genomic DNA from an edited founder mouse is shown. ( b ) Flow cytometry analysis of the indicated immune cell populations in 6-8 weeks old Unc93b1 WT/WT , Unc93b1 PKP/WT , and Unc93b1 PKP/PKP mice. Frequencies of dendritic cells (CD11b + CD11c + MHCII high ) and inflammatory monocytes (CD11b + Ly6c + Ly6G neg ) in lymph nodes are shown. Data points were pooled from four independent experiments. P-values determined by unpaired two-tailed Student's t-test are listed. ( c ) Unc93b1 PKP/PKP mice exhibit signs of emergency granulopoesis in their bone marrow compartment. Flow cytometry analysis of bone marrow from 6-8 week old Unc93b1 WT/WT , Unc93b1 PKP/WT , and Unc93b1 PKP/PKP mice. Gates representing LSK (CD45 + CD3epsilon neg CD19 neg Ly6c neg Ly6G neg Sca-1 high c-Kit high ) and Sca-1 high c-Kit neg cells (CD45 + CD3epsilon neg CD19 neg Ly6c neg Ly6G neg Sca-1 high c-Kit neg ) are indicated and compiled frequencies of Sca-1 high c-Kit neg cells are shown on the right. Bars show mean +- s.d., n=3 biological replicates ( p -value by unpaired two-tailed Student's t-test). ( d ) Representative staining, corresponding to compiled results shown in Fig. 4c , of anti-nuclear antibodies (ANA) using sera from the indicated mouse a