AM31718PU-N

antibody from OriGene

Targeting: RHOA ARH12, ARHA, Rho12, RHOH12

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM31718PU-N

Provider

OriGene

Product name

RHOA mouse monoclonal antibody, clone 1B12, Purified

Antibody type

Monoclonal

Description

RHOA mouse monoclonal antibody, clone 1B12, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

RHOA

Isotype

IgG

Antibody clone number

1B12

Vial size

50 µg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot analysis of RHOA expression in HL-60 cell lysate.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot analysis of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to RHOA on HeLa cell at 10 ug/ml.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Validation comment

IHC