Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000387-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000387-M04, RRID:AB_547699
- Product name
- Mouse Monoclonal RhoA Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RHOA - ras homolog gene family, member A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Cellular uptake of extracellular vesicles is mediated by clathrin-independent endocytosis and macropinocytosis.
RhoA is associated with invasion and poor prognosis in colorectal cancer.
Relationship of RhoA signaling activity with ezrin expression and its significance in the prognosis for breast cancer patients.
Costa Verdera H, Gitz-Francois JJ, Schiffelers RM, Vader P
Journal of controlled release : official journal of the Controlled Release Society 2017 Nov 28;266:100-108
Journal of controlled release : official journal of the Controlled Release Society 2017 Nov 28;266:100-108
RhoA is associated with invasion and poor prognosis in colorectal cancer.
Jeong D, Park S, Kim H, Kim CJ, Ahn TS, Bae SB, Kim HJ, Kim TH, Im J, Lee MS, Kwon HY, Baek MJ
International journal of oncology 2016 Feb;48(2):714-22
International journal of oncology 2016 Feb;48(2):714-22
Relationship of RhoA signaling activity with ezrin expression and its significance in the prognosis for breast cancer patients.
Ma L, Liu YP, Zhang XH, Geng CZ, Li ZH
Chinese medical journal 2013 Jan;126(2):242-7
Chinese medical journal 2013 Jan;126(2):242-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M04), clone 1B12. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in HL-60 (Cat # L014V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RhoA Antibody (1B12) [H00000387-M04] - Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B12) [H00000387-M04] - Analysis of monoclonal antibody to RHOA on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RhoA Antibody (1B12) [H00000387-M04] - Analysis of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human lymphoma tissue.Antibody concentration 5 ug/ml