M00207-1

antibody from Boster Biological Technology

Targeting: RHOA ARH12, ARHA, Rho12, RHOH12

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00207-1

Provider

Boster Biological Technology

Product name

Anti-Rho A + B + C Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for RHOA detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat RHOA information: Molecular Weight: 21768 MW; Subcellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex . Midbody. Cell projection, lamellipodium . Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DGF-18

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Longitudinal genomic alternations and clonal dynamics analysis of primary malignant melanoma of the esophagus.

Li J, Guan W, Ren W, Liu Z, Wu H, Chen Y, Liu S, Quan X, Yang Z, Jiang C, He J, Xiao X, Ye Q
Neoplasia (New York, N.Y.) 2022 Aug;30:100811

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Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress.

Zhao C, Lin M, Pan Y, Yu B
Gastroenterology research and practice 2018;2018:9252984

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody (M00207-1)RHOA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RHOA Antibody (M00207-1)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image