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LearnNB600-258
antibody from Novus Biologicals
Targeting: BCL11A
BCL11A-L, BCL11A-S, BCL11A-XL, CTIP1, EVI9, HBFQTL5, ZNF856
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
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- Product number
- NB600-258 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-258, RRID:AB_10000559
- Product name
- Rabbit Polyclonal Ctip1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references BCL11A represses HBG transcription in K562 cells.
Chen Z, Luo HY, Steinberg MH, Chui DH
Blood cells, molecules & diseases 2009 Mar-Apr;42(2):144-9
Blood cells, molecules & diseases 2009 Mar-Apr;42(2):144-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ctip1 Antibody [NB600-258] - Whole cell lysate (5, 15 and 50 ug) from Ramos cells, probed with Ctip1 Antibody diluted at 0.4 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ctip1 Antibody [NB600-258] - Detection of Human Ctip1 (Bcl11a) by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from Ramos cells. Antibodies: Affinity purified rabbit Ctip1 Antibody NB600-258 used at 0.4 ug/ml. Detection: Chemiluminescence with exposure times of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Ctip1 Antibody [NB600-258] - Whole cell lysate (20% of IP loaded) from Ramos cells, immunoprecipitated by (NB600-258-1) at 3 ug/mg lysate and detected at a dilution of 1 ug/ml. Ctip1 was also immunoprecipitated by prior lot NB600-258-2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Ctip1 Antibody [NB600-258] - Detection of human Ctip1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells. Antibodies: Affinity purified rabbit Ctip1 Antibody NB600-258 used for IP at 6 ul/mg lysate. Ctip1 was also immunoprecipitated by rabbit anti-Ctip1 antibodies (NB600-259, NB600-261) and rabbit anti-Ctip1 recombinant monoclonal antibody [BLR073G] (NBP2-78710). For blotting immunoprecipitated Ctip1, NBP2-78710 was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.
